Mesenchymal stem/stromal cells (MSCs) are known to secrete wound healing factors. However, the delivery of the MSCs and their secretomes remains a barrier to the development of a successful regenerative medicine. Therefore, we examined the content and paracrine activity of serum free MSC conditioned media (MSC CM) generated in routine tissue culture and when cultured on a plasma polymerized membrane previously used clinically in cell therapies for skin wounds.