As a 'medicine food homology' herbal medicine, Fisch. has been consumed widely in the pharmaceutical and food industries. In this paper, seventeen known compounds () were isolated from the ethyl acetate fraction of roots. Their structures were elucidated as isoliquiritin , liquiritigenin , liquiritin , ononin , quercetin , 2,4-dihydroxybenzoic acid , isoliquiritigenin , kaempferol , rutin , naringenin , isolicoflavonol , licoflavonol , β-sitosterol , p-hydroxyphenethylanisate , 6,8-diprenylgenistein luteolin and p-hydroxybenzoic acid . Among them, compounds and were verified with significant α-glucosidase inhibitory activity with IC of 1.9 and 2.6 M for the first time, respectively. Compounds , , and exhibited distinctive antioxidant efficacies. Additionally, the structure-activity relationship analysis has been discussed. Herein, this paper highlights that the ethyl acetate fraction of is a potentially natural source for exploring antioxidant and antidiabetic constituents.